It’s everyones favorite plasmid! PGlo plasmid! This is one of our favorite places to stash GFP for insertion into bacterial DNA. Represented here, we have Ara C which is necessary to make sure the plasmid inserted correctly by utilizing Arabinose in the plating to form a complex, promoting the expression of the GFP. Beta Lactamase is a resistance gene, necessary for countering the antibiotic on the plate medium. The bacteria will be unable to grow on antibiotic medium without this gene, so it is necessary to ensure that the plasmid was inserted and properly coded into the genome. The Ori site, is the origin of replication site necessary in *most* plasmids for replication of the DNA to occur. I say most, because biology is amazing, and some entities like viruses have their own method of replication.
The GFP is detachable, and I will likely add its restriction enzymes there, showing that if you want to isolate just the GFP and you have your own method for ensuring its addition to a DNA sequence, you can use restriction enzymes which cut at specific points in the plasmid to release certain genes. pGlo is designed particularly with this in mind, so GFP is easily removable without disrupting the rest of the plasmid.
In short: my jewelry might accidentally teach you something.